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Fig. S4
High magnification in the adult TeO of transversal polyclones generated from her4-positive RG at 5dpf and 15dpf, revealed using the TetOn system in her4H2a-mCherry, T(5dpf) (A) and her4H2a-mCherry, T(15dpf) (B) fish induced with 9TB. Horizontal sections stained for H2A-mCherry and the RG marker GS, and counterstained with DAPI (Panels 1 and 2: single channels, panels 3: merge), observed under confocal microscopy. Note that these polyclones cover the entire transversal extent of the PGZ and include RG cells (yellow arrows). Scale bars: 50μm.
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Reprinted from Developmental Biology, 420(1), Galant, S., Furlan, G., Coolen, M., Dirian, L., Foucher, I., Bally-Cuif, L., Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain, 120-135, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.