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Fig. S4
BMP4, C2C12 deletions, and ISL controls.
A) ISH analysis of prim-25 stage wildtype and wdr68 mutant animals revealed no differences in expression of bmp4. B) C2C12 wildtype and deletion subline sequences at the targeted locus in exon-5. The yellow highlight indicates the guide RNA target sequence followed by the TGG PAM sequence. C and D) ISH analysis for hand2 expression. C) ISL-treated wildtype sibling. D) ISL-treated mutant sibling lacking rescue. E and F) ISH analysis for dlx2 expression. E) ISL-treated wildtype sibling. F) ISL-treated mutant sibling lacking rescue.
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