ZFIN Image: Alvarado et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Phenotypes and distributions observed in ectopic GFP-Wdr68 fusion protein expressing embryos and control siblings.

A-F) ventral views of 5dpf alcian blue stained cartilages from embryos raised at 28.5°C. A) heat shocked wildtype sibling displaying normal cartilages. B) heat shock induced Tg(hsp70l:GFP-wdr68) overexpression yielded no discernible cartilage phenotype. C) wdr68-MO injected animal showing the mild M-PQ joint fusion phenotype. D) wdr68-MO injected animal showing the severe loss of M and PQ phenotype. E) wdr68-MO injected heat shock induced Tg(hsp70l:GFP-wdr68) animal showing rescued normal M and PQ cartilages. F) wdr68-MO injected heat shock induced Tg(hsp70l:GFP-wdr68) animal showing rescued mild M-PQ joint fusions. G) plot of the distribution of phenotypes observed in a representative experiment on wdr68-MO injected Tg(hsp70l:GFP-wdr68) animals.

Acknowledgments

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