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Fig. 3
Effects of pr130 deletion on mortality and cardiac development. (A,B) pr130-/- embryos develop pericardial edema (arrow). A, WT; B, pr130-/-; (C) Survival curves; (D) Whole-mount in situ hybridization (WISH) using the heart probe cmlc2. Mutants showed a higher proportion of no-looping (arrows) compared with WT which showed normal heart looping; (E) Percentage of abnormal and normal heart looping. n = 100 embryos analyzed per panel. The symbols ** in the bar chart represent significant differences (p < 0.01).
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