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Fig. 4
Effects of CDK9 inhibition on apoptotis. Lower panels – Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunostaining. Zebrafish embryos (Wik, wild type strain) at 72 hpf injected with CDK9-targeting morpholino or continuouly exposed to Flavopiridol 3μM showed significant increase in the appearance of apoptotic bodies (number of TUNEL positive nuclei) compared to control, counted in the trunk region and reported in the scatter graph. At least n = 12 embryo per group; data were statistically analyzed by student t-test; *** = ≤0.001.
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