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Fig. 6
Alterations in heat shock protein expression following HSF1 knockdown.
A: Representative Western blots, and quantitative analysis of Hsp70 and Hsp27 expression. Expression was analyzed in 48 hpf embryos injected with control (CN MO) or HSF1 antisense (HSF1 KD) morpholinos incubated at control temperatures or subjected to heat shock preconditioning (PC). Values represent average data from four independent experiments. Error bars are the SD, symbols indicate a significant difference from CN-MO injected embryos that were not preconditioned (*) and from CN-MO injected embryos that were preconditioned (Ŧ–). B: Representative Western blots and quantitative analysis of Hsp27 and Hsp70 expression after a 100 min hypoxia treatment. Embryos were injected with HSF1 antisense MO (HSF1 KD) or a control MO (CN). Bars represent data collected from three independent experiments. Error bars are the SEM.