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Fig. 8
nlk and wnt8 ORF2 MOs enhance the phenotype of wnt11. Morpholinos were injected in the combinations listed in the table. wnt8.1 refers to wnt8 ORF1 and wnt8.2 refers to wnt8 ORF2. Wnt11 and strabismus morpholinos were injected at a concentration of 0.5 ng/nl, whereas nlk, wnt8 ORF1 and wnt8 ORF2 MOs were injected at a concentration of 1 ng/nl. (A) Embryos were scored at 48 hours and placed into five phenotypic classes based on the severity of the cyclopia phenotype (Classes I-V, according to Marlow et al.), and the Cyclopia Index (Marlow et al., 1998) was calculated for each experiment. (B-E) Embryos were injected with nlk, wnt11, or both morpholinos, fixed at 100% epiboly, and stained with probes for tbx6 and gsc. The anterior extent of the gsc domain is marked with an arrowhead.