ZFIN Image: Thorpe et al., 2004, Fig. 3

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Fig. 3

nlk RNA represses anterior neural fate in a wnt8-dependent manner. (A-F) 90-95% epiboly, lateral view, dorsal to the right. Embryos are stained with a mix of opl and tbx6 probes. Injection of 200 pg nlk RNA (B) or 10 pg wnt8 RNA (C) represses expression of opl (arrowheads) in the prospective telencephalon, compared with wild type (A). Injection of 1 ng each of wnt8 ORF1 and ORF2 morpholinos (MOs) causes a posterior shift of opl expression towards the margin (D, arrowhead). Co-injection of a control MO with nlk RNA has no effect on opl (E), whereas injection of wnt8 MOs restores opl expression when co-injected with nlk RNA (F). (G,H) Twenty-four-hour embryos, lateral view, showing rescue of eyes in nlk RNA + wnt8 MOs (G) versus nlk RNA + control MO (H).

Acknowledgments

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