ZFIN Image: Bernut et al., 2016, Fig. 4

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Figure Caption

Fig. 4

M. abscessus infections are associated with a massive neutrophil mobilization.

(A-D) Confocal live imaging of Tg(mpx:eGFP) larvae after iv (A and D) or intramuscular (B and C) infection with ≈100 Mabs R (tdTomato). (A-B) Time-lapse of neutrophils recruitment in caudal vein (A), monitored from 20 mpi to 110 mpi, or into muscle (B) monitored from 10 mpi to 150 mpi. Scale bars, 20 μm. (C) Recruited neutrophils phagocytizing individual mycobacteria. Scale bar, 5 μm. (D) Neutrophils (arrows) and presumptive macrophages (*), containing mycobacteria. Scale bar, 20 μm. (E) Number of infected neutrophils and macrophages counted in CHT at 4 hrs post intravenous infection with both Mabs variants (≈150 CFU, three independent experiments, horizontal lines indicate mean values). M, macrophage; N, neutrophils. Statistical significance was determined by one-tailed unpaired Student’s t test. (F) Confocal imaging of neutrophil-bacteria interactions in the context of R-abscess. Scale bars, 20 μm. See also S1 Movie.

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Acknowledgments

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