ZFIN Image: Bernut et al., 2016, Fig. S6

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Figure Caption

Fig. S6

Absence of TNF signaling promotes macrophages death.

(A and B) WT or tnfr morphants Tg(mpeg1:mCherry-F) were iv infected with either R or S variants of Mabs expressing E2 Crimson (≈150 CFU) and stained for dead macrophages with acridine orange (AO). (A) Representative microscopy showing the dead macrophages in 2 dpi R-infected embryos. Scale bars, 80 μm. (B) Number of dead infected macrophages evaluated using confocal microscopy at 2 dpi. Each symbol represents individual embryos and horizontal lines indicate mean values. (C) WT, il8 or csf3r morphants Tg(mpeg1:mCherry-F) were iv infected with either R or S variants (≈150 CFU) and stained for dead macrophages with acridine orange (AO). Statistical significance was determined by one-tailed Student’s t test (B) or Pearson correlation (C). Results are presented as the average number from two experiments.

Acknowledgments

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