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Fig. S5
ROS production by hematopoietic cells following systemic Mabs infection.
Evaluation of ROS induction in infected embryos using CellROX Green fluorescent. Tg(mpeg1:mCherry-F) (A) or Tg(LysC:DsRed) (B) were iv infected with E2 Crimson-expressing Mabs (R variant) and monitored for the ROS detection. (A) Confocal microscopy of agglomerates of hematopoietic cells display ROS production. Scale bar, 15 μm. Arrows indicate ROS-positive macrophages. (B) Mobilization of neutrophils close to infected tissue with agglomerates of hematopoietic cells producing ROS is revealed by confocal microscopy. Scale bar, 20 μm.
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