ZFIN Image: Bernut et al., 2016, Fig. 6

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Figure Caption

Fig. 6

TNF signaling is required for neutrophil recruitment.

(A-D) WT or tnfr morphant Tg(mpx:eGFP) larvae were injected in the otic cavity (A-B) or hindbrain (C-D), with both variants of Mabs (tdTomato, ≈100 CFU, two or three experiments) and imaged at 3 hpi. Representative images (A and C) and number (B and D) (horizontal lines indicate the mean values) of neutrophils at the infection site. Scale bars, 100 μm. (E and F) Confocal image of neutrophils surrounding a 3 dpi R-cord (E) (Scale bars, 50 μm) or a 5 dpi R-abscess (F) (Scale bars, 100 μm) in iv infected WT or tnfr morphant Tg(mpx:eGFP) embryos. Statistical significance was determined by one-tailed Student’s t test (B and D). See also S2–S5 Movies.

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Acknowledgments

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