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Fig. S4
Heat-shock activation of hs:gsc during gastrulation or otic development. (A–C) Whole-mount live images at 24 hpf (dorsal up, anterior left) show the phenotypic effects of a 30-min, 39 °C heat shock initiated at 6 hpf in control and hs:gsc embryos. Activation of hs:gsc under these standard heat-shock conditions dorsalized the embryos to varying degrees (B and C). (D–I) Whole-mount images (dorsal up, anterior left) showing gsc expression in controls and hs:gsc embryos. Embryos were fixed and stained at indicated times after the end of a 60-min heat shock initiated at 22 hpf. Activation of hs:gsc at 22 hpf led to a strong increase in the gsc level by the end of the heat-shock period (E). The level of gsc expression was maximal at 23.5 hpf (F) but declined rapidly thereafter, returning to normal by 25 and 26 hpf (H and I).