ZFIN Image: Grainger et al., 2016, Fig. 3

Image

Figure Caption

Fig. 3

Wnt9a Is Required for HSPC Formation

(A and B) WISH for wnt9a at 16.5 hpf, 19 hpf, and 24 hpf (A). Scale bars, 0.2 mm; representative images of n = 15. Imaged area is shown in (B).

(C and D) Representative images of kdrl:mCherry; cmyb:eGFP injected as listed (C). HSPCs per millimeter of aorta are quantified in (D). Scale bars, 30 μm.

(E) WISH for cmyb in 4 days post fertilization (dpf) fish treated with 0.1 ng or 1 ng of wnt9a MO. Scale bars, 0.2 mm.

(F) Wnt9a mutants were generated by injection of guide RNA targeting the last portion of the first exon.

(G and H) Expression of cmyb was examined by WISH in 36 hpf WT, wnt9a^+/Δ and wnt9a^Δ/Δ- zebrafish (G) and quantified in (H).

Scale bars, 100 μm. ^∗p < 0.05; ^∗∗∗p < 0.001; n.s., not significant. Error bars represent SD. See also Figure S3.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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