ZFIN Image: Widziolek et al., 2016, Fig. 3

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Figure Caption

Fig. 3

Pg W83 disseminates rapidly in zebrafish larvae and is able to cross the vascular barrier to invade surrounding tissues.

Confocal images of kdrl:memRFP transgenic zebrafish larvae at 1 hpi following systemic infection with PBS control (a) or fluorescein-labelled 5 × 10⁴CFU Pg W83 (b,c). Fluorescent Pg W83 was co-localised to the red-labelled endothelium (white arrowhead in c) and cross the vasculature into the tissue (blue arrowheads in c). Light sheet image of fluorescein-labelled Pg W83 adherent to the heat tissues of zebrafish larvae (d). Pg are rapidly phagocytosed; high-power image of phagocytosed Pg within a phagocyte (red, black arrowhead) and non-phagocytosed Pg (green, orange arrowhead) (e). Scale bar = 20 μm. Images are representative of two independent experiments showing similar findings.

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Acknowledgments

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