ZFIN Image: Li et al., 2015, Fig. 5

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Figure Caption

Fig. 5

impdh1a knock-down leads to pigmentation defects and smaller eyes.

(A,D,G,J,M): Coronal planes through 5 dpf larval eyes. Xanthophores (yellow arrow) and iridophores (red arrow) were reduced significantly in impdh1a morphants. (B,E,H,K,N): Transverse histological sections of impdh morphants. The eye length along the dorsal/ventral axis was much shorter in impdh1a morphants. There was no apparent phenotype in retinal cell types between WT, control and the three impdh morphants; (C,F,I,L,O): Dorsal images of 5 dpf larvae showed that melanophores (black arrow) covered a larger area of the head in impdh1a morphants. Anterior-posterior eye length (P) and melanocyte covered area (Q) were measured and compared using a Student’s t-test (unpaired, two tailed; *** p<0.001; ** p<0.01; * p<0.05). At least ten larvae were tested in each group. Error bars represent the standard error of mean (SEM) among independent biological replicates. GCL: ganglion cell layer, INL: inner nuclear layer, IPL: inner plexiform layer, OPL, outer plexiform layer, PR: photoreceptor layer, RPE: retinal pigment epithelium. Scale bars, 50μm.

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Phenotype details

Acknowledgments

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