ZFIN Image: Jia et al., 2015, Fig. S9

Image

Figure Caption

Fig. S9

Analysis of total protein and polysome assembly in WT and kri1l^cas002 embryos. (A-C) Total protein was extracted from lysates of deyolked whole embryos (n>20) and then measured by BCA (bicinchoninic acid) protein assay. The protein synthesis was impaired at 3 dpf (p=0.0003), 4 dpf (p<0.0001) and 5 dpf (p<0.0001). Error bars represent standard error of the mean (SEM). ***, P≤0.001 (Student t test). (D) Representative polysome fractionation analysis performed on WT and kri1l^cas002 embryos at 3 dpf demonstrates reduced levels of 40 S ribosomal subunits, 80 S monosomes and polysomes; and excessive 60 S subunits in kri1l^cas002 larvae compared to that in WT.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Res.