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Fig. S3
Sequences and characterization of zebrafish katnb1 mutant alleles.
(A) Multiple sequence alignment of the human, mouse and zebrafish Katnb1, showing a high level of conservation of the protein sequence between the species.
(B) Summary of identified TALEN-induced mutations in zebrafish katnb1. Genomic region surrounding the exon 6 - intron 6 boundary in zebrafish is highlighted. TALEN recognition sites are marked with blue boxes; exonic sequence (capital letters); intronic sequence (lowercase). Wild-type sequence is listed above and the sequences of identified alleles are detailed below; deleted base pairs (red dashes); inserted base pairs (red letters). The effect on the genomic sequence is summarized to the right of each sequence: - deletion; + insertion; presence (+) or absence (Δ) of the splice site; allele names; frequency of the allele in 288 fish analyzed.
(C) Progeny from homozygous female carriers of katnb1 mutations develop normally through epiboly. Summary of phenotypes appearing at 24 hpf ranging from mild microcephaly, anencephaly and disrupted embryos.