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Fig. 6
A small population of differentiating neurons express phox2bb and ret at later stages of neuronal differentiation. A and C show confocal images of cross-sections through the trunk. (A) At 84 hpf, ret mRNA (red) colocalizes with Elavl (blue) and phox2bb (green). Insets show close-ups of outlined cell, overlay, phox2bb, ret mRNA, Elavl (from left to right). Note that phox2bb refers to phox2bb:EGFP expression as described in the text. (B) At 84 hpf, quantification in percent of phox2bb, ret and Elavl colocalization in the three regions analyzed. (C) At 84 hpf, sox10 mRNA (red) does not colocalize with Elavl positive (blue), phox2bb positive (green) cells. Insets show close-up of outlined cell, overlay, phox2bb, sox10 mRNA, Elavl (from left to right). (D) At 84 hpf, quantification in percent of phox2bb, sox10 and Elavl colocalization in the three regions analyzed. The dotted line between the measured values does not show the continuous percentage of coexpression between the anterior, mid and posterior values, but is drawn to facilitate visualization of coexpression trends. ie, intestinal epithelium. Scale bar = 10 μm.