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Fig. 5
A small population of differentiating neurons express phox2bb and ret. A, C, E, and G show confocal images of cross-sections through the trunk. At 54 (A) and 60 (C) hpf, ret mRNA (red) colocalizes with Elavl (blue) and phox2bb (green). Insets show close-ups of outlined cell, overlay, phox2bb, ret mRNA, Elavl (from left to right). Note that phox2bb refers to phox2bb:EGFP expression as described in the text. At 54 (B) and 60 (D) hpf, quantification in percent of phox2bb, ret and Elavl colocalization in the three regions analyzed. At 54 (E) and 60 (G) hpf, sox10 mRNA (red) does not colocalize with Elavl positive (blue), phox2bb positive cells (green). Insets show close-up of outlined cell, overlay, phox2bb, sox10 mRNA, Elavl (from left to right). At 54 (F) and 60 (H) hpf, quantification in percent of phox2bb, sox10 and Elavl colocalization in the three regions analyzed. The dotted line between the measured values does not show the continuous percentage of coexpression between the anterior, mid and posterior values, but is drawn to facilitate visualization of coexpression trends. ie, intestinal epithelium. Scale bar = 10 μm.