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Fig. S6
Multiplexed in situ HCR in whole-mount zebrafish larvae (D. rerio). (a) Individual channels from 4-channel confocal images. For each of three replicate larvae, a representative optical section was selected for each channel based on the expression depth of the corresponding target mRNA. (b) Pixel intensity histograms for Signal + Background (pixels within solid boundary) and Background (pixels within dashed boundary). For each image, the total number of pixels within solid and dashed boundaries is the same. Larvae fixed: 5 dpf. Scale bar: 25 μm.
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