Fig. S3

Fig. S3

Vclb^del17 mutant exhibits the same phenotype as v12 mutant. (A) In vclb^del17 mutants, a17-bp deletion in vclb gene was created using CRISPR/Cas9 technology. The deletion removes a BglII site in vclb gene used for genotyping. (B) At 3dpf, vclb^del17 mutant shows similar pericardiac edema and pleural effusion in v12 mutant. (C) At 30 dpf, vclb^del17 mutant has craniofacial defects resembling v12 mutant. (D-G) vclb^del17 mutant shows increased epicardial expansion and Aldh1a2 expression at 40 dpf (E)and up-regulation of pERKin the endocardium at 55 dpf(G), both are similar to v12 mutant. Wildtype sibling controls are shown at the same stages (D, F). MF20 labels cardiomyocytes and DAPI labels nuclei. (H-M) vclb^del17 mutant shares similar coronary vessel defects with v12 mutant as indicated with a Tg(fli1a:EGFP) transgenic reporter, which labels endothelial cells. (N-N',O-O') Tg(tcf21:dsRed) transgenic reporter, which labels epicardial cells. vclb^del17 mutant shows similar over-proliferation of epicardial derived cells in v12 mutant. Scale bar:20μm(D-G, L-O'), 100μm(H-K).