ZFIN Image: Marín-Juez et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Expression of Vegf family members in zebrafish CMs. (A) FACS isolation of EGFP⁺ CMs from uninjured Tg(myl7:EGFP) adult zebrafish hearts. Cellular debris were excluded, and the gated population of EGFP⁺/propidium iodide (PI)^- cells was sorted. (B) qPCR analysis of myl7 (as a positive control), vegfaa, vegfab, vegfc, vegfr1, vegfr2, vegfr3, and six3b (as a negative control) expression in FACS-sorted myl7:EGFP⁺ CMs. (C) qPCR analysis of vegfab expression at 6 hpi and 1, 2, 3, and 5 dpi. Data (mean ± SD) are shown relative to their respective sham controls. *P < 0.05 (n = 4, each replicate consists of a pool of 4 ventricles).

Acknowledgments

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