ZFIN Image: Gutzman et al., 2015, Fig. 7

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Figure Caption

Fig. 7

Non-muscle myosin IIA and IIB protein localization. (A-C) NMIIA and NMIIB antibody staining in 24 ss embryos injected with control MO. (A) NMIIA antibody (green), (B) NMIIB antibody (red), (C) overlay of A and B. (D) Overlay of NMIIA and NMIIB immunostaining in a 24 ss mypt1 MO injected embryo. (E) NMIIA staining with myh10 MO knockdown. (F) NMIIB staining with myh9b MO knockdown. (G-H) No primary control from Alexa 488 and Alexa 555 secondary antibodies. Results are representative of at least three independent experiments. Control MO, n=8; mypt1 MO, n=7; myh10 MO, n=14; myh9b MO, n=19; Alexa 488 no primary control, n=5; Alexa 555 no primary control, n=5.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 397(1), Gutzman, J.H., Sahu, S.U., Kwas, C., Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis, 103-15, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.