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Fig. 3
The Traf6-MyD88 pathway is involved in up-regulation of miR-146a and miR-146b expression levels upon infection. (A) Traf6-dependent miR-146a/b induction. Embryos were injected with traf6 morpholino (MO) or a mismatch morpholino (MM) as a control. Embryos were infected at 28 hpf with S. typhimurium (inf) or mock-injected with PBS and samples were collected at 8 hpi. (B) MyD88-dependent miR-146a/b induction. Mutant (myd88-/-) and wild type siblings (myd88+/+) were infected with S. typhimurium at 28 hpf followed by sample collection at 8 hpi. Expression levels in both experiments were determined by TaqMan qPCR and relative expression levels are shown with the mock control set at 1. Data are the mean ± SEM of three replicate sample sets. Asterisks indicate significant differences (*, P < 0.05; **, P <0.01) tested by one-way ANOVA analysis with Tukey’s method as post-hoc test.