ZFIN Image: Sicca et al., 2016, Fig. 6

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Figure Caption

Fig. 6

In vivo modelling of Kir4.1 mutations in zebrafish.

(a) Transient kcnj10a knockdown zebrafish show macroscopic abnormalities in organ development compared to wild-type (WT). In morphant embryos, the pronephric duct is visible because it is dilated (black arrow); the swim bladder is not visible (white arrow). (b-e) Number of spontaneous tail flicks (registration time 30sec) seen in WT and mutant 30 hpf embryos. Values are expressed as percent of flicks counted in uninjected embryos. Embryos injected with MO (b-e), and with equimolar amount of MO and either R18Q (b), V84M (c), and R348H (d) mRNA show an increased rate of spontaneous contractions, compared to uninjected embryos (b-e), and to either MO+WT (b-e) and MO+R271C (e) mRNA injected embryos. **p < 0.01; ***p < 0.001.

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Acknowledgments

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