ZFIN Image: Nissim et al., 2016, Fig. S5

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Figure Caption

Fig. S5

Effects of Cpd3 exposure on pancreas development. A) Endocrine pancreas cells (black arrows) expressing islet1 are present by in situ hybridization in embryos treated with Cpd3 from 12 - 72 hpf. B) δ-cells expressing somatostatin (sst) (black arrows) are present by in situ hybridization in embryos treated with Cpd3 from 12 - 72 hpf. C) Quantification of exocrine pancreas size by area of trypsin expression at 72 hpf. 200 µM Cpd3 treatment elicits a robust reduction in pancreas size in heterozygote nr5a2^+/oz3 embryos relative to control nr5a2^+/oz3 siblings and control nr5a2^+/+ embryos. (n > 5 per group; for control nr5a2^+/+ vs. Cpd3 treated nr5a2^+/+, p = 0.2498; for control nr5a2^+/oz3 vs Cpd3 treated nr5a2^+/oz3, p = 0.0027; for control nr5a2^+/+ vs. Cpd3 treated nr5a2^+/oz3, p = 0.0003; ordinary one-way ANOVA with Tukey’s multiple comparisons test).

Acknowledgments

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Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.