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Fig. S3
Additional nr5a2oz3 mutant phenotyping A) Double in situ hybridization for insulin (brown) and nr5a2 (purple) from 48 - 96 hpf. nr5a2 and insulin are independently expressed from 72 - 96 hpf. B) In situ hybridization for mature hepatocyte marker transferrin A demonstrates that nr5a2oz3/oz3 embryos have a reduced liver size. C) In situ hybridization for fabp10a at 68 hpf reveals that fabp10a+ hepatocytes fail to form in the liver region of nr5a2oz3/oz3 embryos (red arrows). D) nr5a2oz3/oz3 embryos have normal prox1 expression in the liver and pancreas regions at 30 hpf.
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Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.