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Fig. 4
A subset of hepatopancreas progenitors require nr5a2 for specification. (A) nr5a2oz3/oz3 mutants have reduced prox1 expression in the liver (red arrow) and pancreas (blue arrow) buds relative to nr5a2+/+ controls at 48 hpf. (B) Quantification of prox1 expression area in the combined liver and pancreas buds (n>3 per group; for nr5a2+/+ vs. nr5a2+/oz3, p=0.1502; for nr5a2+/+vs. nr5a2+/oz3, p=0.0016; unpaired t-test). (C) In situ hybridization reveals consistent decreased hnf4α expression in the hepatoblasts (red arrow) of nr5a2oz3/oz3 embryos. (D) Quantification of hnf4α expression area in the intestine and liver buds shows significant expression reduction in nr5a2oz3/oz3 embryos relative to nr5a2+/+ and nr5a2+/oz3 groups (n>3 per group; for nr5a2+/+vs. nr5a2oz3/oz3, p=0.0014; for nr5a2+/oz3vs. nr5a2oz3/oz3, p<0.0001; unpaired t-test). (E) The size of the hhex liver bud (red arrow) is reduced in the nr5a2oz3/oz3 embryos compared to nr5a2+/+ siblings, while the pancreas bud (blue arrow) is largely unaffected. (F) In situ hybridization for foxa3 endoderm marker consistently demonstrates delayed gut looping, liver (red arrow), and pancreas (blue arrow) budding in the nr5a2oz3/oz3 embryos at 32 hpf. (G) The sizes of the foxa3 liver (red arrow) and pancreas (blue arrow) buds are consistently reduced in the nr5a2+/oz3 and nr5a2oz3/oz3 mutant populations at 48 hpf.
Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.