ZFIN Image: Zhang et al., 2012, Fig. S1

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Figure Caption

Fig. S1

Morpholino Oligonucleotides Blocking agrp Splicing Reduce Normal Somatic Growth in Larval Zebrafish.

(A) Bar diagram showing the targets and predicted consequences of two morpholino oligonucleotides designed to block splicing of the agrp mRNA. (B) Efficiency of each antisense MO was confirmed by RT-PCR at 2dpf. Red arrows show predicted longer form of agrp RNA with disruption of the first splice donor (E1I1, left panel) and shorter form of agrp RNA resulting from blockade of first splice acceptor (I1E2, right panel), as seen on agarose gel from agrp E1I1 exon donor morphant and agrp I1E2 exon acceptor morphant, respectively. Predicted protein sequences of WT and morphant AgRP proteins are indicated. (C) Body lengths of fish injected with standard control MO, E1I1 MO and I1E2 MO were measured with a micrometer at 5dpf. Bars indicates mean±s.e.m. Results were analyzed by one way ANOVA followed by Tukey post test. (n=36, 33 and 42. ***, p<0.001).

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Cell Metabolism, 15(2), Zhang, C., Forlano, P.M., and Cone, R.D., AgRP and POMC neurons are hypophysiotropic and coordinately regulate multiple endocrine axes in a larval teleost, 256-264, Copyright (2012) with permission from Elsevier. Full text @ Cell Metab.