Fig. S5
Localization by real-time PCR of zebrafish tac3a, tac3b, tac3ra, and tac3rb mRNA in various tissues. The relative abundances of the mRNAs were normalized to the amount of elongation factor 1-α (ef1α) by the comparative threshold cycle method, where the comparative threshold reflects the relative amount of the transcript. Ant. Intest+ panc., anterior intestine and pancreas; post. Intes, posterior intestine. We found low levels of mRNA expression of all four transcripts in the liver, retina, and adipose tissue. However, relatively high mRNA levels of tac3a and tac3rb were expressed in the gills, tac3a in the posterior intestine and tac3ra in the muscle.