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Fig. 3
Hypoxia increases the expression of hif1-α and twist in zebrafish.
Zebrafish at 48 hpf were treated with or without 21% O2, 5% O2 or indicated concentration of DFX for 1 day and the expression of hif-1α, twist1a, twist1b, twist2 and twist3 genes was assayed by quantitative RT-PCR. Results are shown as the relative expression to ²-actin (mean ± SD) and significance was determined by Student′s t-test. (* p<0.05 and ** p<0.01 versus 21% O2).
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