|
Fig. 2
Comparison of muscle birefingency between wild type and muscle mutants. Muscle fibers are affected in tur (B), slp (D), dus (E) and buf (G) mutants compared to wild type (A,C,F). Optical section through lateral myotomes in a 72-hour wild-type embryo (A) shows muscle fibers in the dorsal half of the somites. In tur mutants, similar to all mutants in the phenotypic groups A2 and A3, birefringency appears reduced or disorganized (B). In slp mutants about 20% fewer muscle fibers are visible (D) and in dus mutants (E) only residual fibers can be seen (arrowheads), compared to wild-type embryos at 60 hours (C). In wild-type muscle tissue (F) segmental organization of the sarcomeres is visible; the arrowheads indicate three individual units. In buf mutants sarcomeric organization is perturbed (G). Sarcomeres appear less evenly spaced and reduced in size.