ZFIN Image: Welker et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Analysis of tumor burden in live animals over time. Confocal images superimposed on bright field (anterior to the left) of two representative casper zebrafish transplanted with 50-75 GBM9 cells (A-A′′′,B-B′′′) and a casper animal transplanted with control mNSC cells (C-C′′′) imaged at 2 (A,B,C), 5(A′,B′,C′), 7(A′′,B′′,C′′) and 10 (A′′′,B′′′,C′′′) dpt. Examples of a compact (A-A′′′) and diffuse tumor (B-B′′′) are shown. (D) Tumor burden were quantified using volume measurements of florescence in micrometers cubed. Approximately 50-75 GBM9 cells (green lines) and ~50 mNSC cells (blue lines) were transplanted and followed over time in the same animal. n=8 animals per group. Scale bars: 100 µm.

Acknowledgments

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