ZFIN Image: Ocaña et al., 2012, Fig. S3

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Figure Caption

Fig. S3

Related to Figure 3. Efficiency and specificity of morpholino antisense oligonucleotides.

(A-B) Specificity was assessed by injecting embryos with a morpholino oligonucleotide (MO) against prrx1a or twist1b together with a plasmid containing the sequence of the prrx1a^MO (A) or twist1b^MO (B) in front of the Egfp gene. Efficiency was directly associated with the decrease in EGFP expression compared with that obtained after injection with the corresponding MO, checked at 10-12hpf by immunohistochemistry with a POD-conjugated antibody and developed with DAB (upper panels) and EGFP fluorescence emission (lower panels, fluorescence pictures taken with the same settings). Scale bar: 1 mm.

(C) The efficiency of the prrx1a splice morpholino was assessed in injected embryos by RTPCR amplification with primers from exon1 and 2, which yield a 108 bp fragment; unprocessed mRNA of 2.6 kb long cannot be amplified. The efficiency is directly related to the decrease in the amount of the 108 bp fragment.

Acknowledgments

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Reprinted from Cancer Cell, 22(6), Ocaña, O.H., Córcoles, R., Fabra, A., Moreno-Bueno, G., Acloque, H., Vega, S.,Barrallo-Gimeno, A., Cano, A., Nieto, M.A., Metastatic Colonization Requires the Repression of the Epithelial-Mesenchymal Transition Inducer Prrx1, 709-724, Copyright (2012) with permission from Elsevier. Full text @ Cancer Cell