|
Fig. 4
Three functional lmx1b alleles are required for correct numbers of glutamatergic cells at later developmental stages. Lateral view of zebrafish spinal cord at 48 h (a-g′, i and j), anterior left, dorsal top. in situ hybridization for slc17a6a + slc17a6b (slc17a6) (a-g′) and slc32a1 (i and j). (a′-g′) are magnified views of black dashed box regions in panels (a-g). Columns on left indicate lmx1ba and lmx1bb genotype. Mean number of cells (y-axis) expressing slc17a6 (h) and slc32a1 (k) in spinal cord region adjacent to somites 6-10 at 48 h (x-axis). Square brackets and star in (h) indicates that each of the first three columns is statistically significantly different from each of the last four columns (p < 0.05). Embryo genotype is indicated below graph. Error bars indicate standard error of the mean. Two independent experiments were conducted for (a-g). Cell count results were similar in each replicate. One experiment was conducted for (i and j). Cell count data presented here (h and k) are average values of 4-13 embryos. Precise numbers of embryos counted and p values are provided in Table 5. Scale bar (g) = 50 µm (a-g) and 20 µm (a′-g′) and scale bar (j) = 50 µm (i, j)