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Fig. S1
Phospho-Smad-1/5/9 positive cardiomyocytes were seen throughout the ventricle in ethanol-exposed embryos.
(A-I) 3D reconstruction of confocal sections of phospho-Smad-1/5/9 (A, D, G) and MF20 (B, E, H) double immunostained embryos showed Bmp responsive phospho-Smad-1/5/9 positive cardiomyocytes at the base of the atrium and in the inner curvature of the ventricle in control embryos (A); regionalization of phospho-Smad-1/5/9 positive cardiomyocytes were not evident in ethanol exposed embryos (D, G); A′, D′, G′: magnified images of boxed areas of A, D, G.
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