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Fig. 1
Enhanced angiogenesis in the liver by induction of transgenic Myc expression in TO(Myc) larvae.
Tg(Fli1:EGFP), LiPan and TO(Myc) zebrafish were crossed to generate triple transgenic zebrafish larvae. The liver was labeled by DsRed expression and blood vessel by EGFP expression. Triple transgenic larvae were treated with 30 µg/ml Dox from 3 dpf to 7 dpf and imaged by a confocal microscope. (A) A representative triple transgenic larva without Dox treatment. (B) A representative triple transgenic larva with Dox treatment. (C) Blood vessel density. Blood vessel density is defined by the ratio of blood vessel area (green) over the total liver area (as demarcated by DsRed expression) and measured by online ImageJ software. The quantitative data were based on 10 samples per group. The original magnification was 40x. Standard error bars are indicated and the two groups show significant difference with P-value < 0.05 by unpaired t-test statistical analysis. Scale bars = 100 µm.