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Fig. 3
Whole-mount antibody stainings of embryos for pax6 (30 hour), islet proteins (antipan- islet antibody; 24 hour), and fret43 (24 hour) visualizing the anterior pituitary and epiphysis in wild-type (A-C) and mbl (D-F) embryos. (A,D) Ventral view of the head of embryos stained for pax6 showing a reduction in the number of pax6-positive neurons within the anterior pituitary (arrowhead) of mbl embryos. (B,C,E,F) Dorsal view of the head of embryos stained for islet proteins (B,E) and fret43 (C,F) showing an increase in the number of islet/fret43-positive cells within the epiphysis (arrowhead) of mbl. Anterior to the left.