ZFIN Image: Qiu et al., 2016, Fig. S4

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Figure Caption

Fig. S4

EOS expression pattern in Tg(foxc1b:EOS) transgenic line. This line was identified from the founder fish that was injected with the Tol2(foxc1b:EOS) construct at the one-cell stage. The expression of EOS was driven by a 3.6-kb promoter of the foxc1b gene. (A, B, E) Expression patterns of foxc1b mRNA in wild-type embryos (A and E) and eos mRNA in Tg(foxc1b:EOS) embryo (C and F). The expression of scl (red) and eos mRNAs (black/blue) was detected by double in situ hybridization. The posterior part of the embryos in (C, E and F) was dorsally viewed. The stages were indicated. (D and G-K) Confocal images showing EOS expression in transgenic embryos at indicated stages. Embryos were laterally viewed. (H′) An optical cross section at the position indicated in (H). (J′) An optical cross section was reconstituted from the posterior trunk region of the embryo shown in (J).

Acknowledgments

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