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Fig. S1

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ZDB-IMAGE-160915-32
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Figures for Smith et al., 2011
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Fig. S1

The heart rate and myocardial cell number are unaltered in wkm mutants.

(A) The heart rate is not significantly different in mutant embryos as compared with siblings (n=15) at 50 hpf, indicating no effect of the wkm mutation on pacemaker activity. (B) Myocardial cell counts showed no significant difference between wkm sibling and mutant embryos at 50 hpf, indicating that myocardial cell number does not contribute to the defective looping phenotype of wkm mutants. Counts were performed on confocal z-stacks of wkm mutant and sibling embryos (n=5-6) on a myl7:dsRed transgenic background. Statistical significance was determined by t-test; bars represent mean ± s.e.m.

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