ZFIN Image: Hortopan et al., 2011, Fig. 1

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Figure Caption

Fig. 1

A,B: Gene expression detection of all the genes of interest (gfap included), using (A) reverse transcriptase-polymerase chain reaction (RT-PCR) in 2% ethidium bromide agarose gel electrophoresis (A) and quantitative real-time PCR (qPCR; B). Levels of mRNA, measured by qPCR were normalized to β-act. Error bars indicate ± SEM. Student′s t-test, P < 0.05. bhlhb5, basic helix-loop-helix domain containing, class B, 5; dbx1a, developing brain homeobox 1a; dmbx1a, diencephalon/mesencephalon homeobox 1a; gfap, glial fibrillary acidic protein; her4.2, hairy-related 4.2; hes5, hairy and enhancer of split 5; hoxa5a, homeo box A5a; hoxb5b, homeobox protein (hoxb5b) gene; nxph1, neurexophilin 1; plxnd1, plexin D1.

Figure Data

Expression / Labeling details

Acknowledgments

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