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Fig. S8
Changes in downstream Wnt target gene expression. (A-H) In situ hybridization for Wnt target gene expression in wild-type and rbc3a-MO1-injected embryos. Dorsal views (A-F), lateral views (G, H). Several genes display reduced expression in the NC (arrowheads) in rbc3a-MO1-injected embryos from 10-12 hpf including gbx2 (A, B), snail2 (C, D), twist1a (E, F), and axin2 (G, H). Scale bar, 100 µm. (I) axin2, nmyc, and lef1 show decreased expression by 13 hpf in rbc3a-MO1-injected embryos. Error bars represent triplicate experiments ± SEM. * p<0.05.
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