ZFIN Image: Noonan et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Abnormal phenotype of proximal vhl^-/- pronephric tubules is specifically recovered by HIF2a inhibitor Compound 76 and not by the inactive HIF2a inhibitor Compound 05. (A) Treatment protocol. Tg(ATPase1.a1A4:GFP) vhl^-/- and wt siblings were treated with 1 µM Compound 76, 05 or DMSO vehicle control from 2 dpf to 5.5 dpf. (B) H&E staining of 5.5 dpf vhl^-/- and wt sibling proximal pronephric tubules (red boxes) treated with Compound 76, 05, or DMSO. Magnification is 60x. Scale bars are 0.05 mm long. (C) Quantification of mean lumen diameter length (transverse section) in 5.5 dpf vhl^-/- and wt siblings treated with 76, 05, or DMSO control. (D) Quantification of mean lumen diameter width (transverse section) in 5.5 dpf vhl^-/- and wt siblings treated with 76, 05, or DMSO control. (E) Overall morphology of 5.5 dpf vhl^-/- and wt siblings treated with 76, 05, or DMSO control. Each experiment had at least 5 larvae per sample group and was performed in duplicate. Data represent mean ± s.e.m. *P≤0.05.

Acknowledgments

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