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Fig. 4
Vhl-/- larvae have increased glycogen content in the proximal pronephros compared to wt siblings. 5.5dpf vhl-/- and siblings were fixed and embedded in JB-4 and sectioned with a glass knife. PAS staining was performed with glycogen stained in purple. PAS staining of wt siblings (A-C) and vhl-/- (D-F) proximal pronephric tubules (red boxes). Asterisks indicate the liver, which serves as a positive control. Scale bars: 0.05mm. Each experiment had at least five larvae per sample group and was performed in duplicate.
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Full text @ Dis. Model. Mech.