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Fig. S4
Generation of tbx6:ggff transgenic fish. (A) Schematic diagram showing construction of the transgene. tol2 and ggff-pA-kan cassette were introduced into CH211-136M16 by using homologous recombination in E.coli. (B,C) The expression pattern of ggff mRNA was almost identical to that of endogenous tbx6 mRNA at the 11-somite stage. (D) The expression of GFP-GFF fusion protein (GGFF) was assessed by immunostaining using anti-GFP antibody. The expression GGFF was detected in the anterior PSM and mature somite region at the 11-somite stage. (E) mcherry expression in tbx6:ggff/uas:gfp-2a-h2a-mcherry double transgenic embryo at the 11-somite stage. Lateral and dorsal views of embryos are shown in B-E and B′-E′, respectively.