ZFIN Image: McKeown et al., 2012, Fig. 7

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Fig. 7

Motor neurons in tnt mutants exhibit increased burst duration. (A,B) Supraspinal electrical stimulation was used to elicit bursts in CaP motor neurons to evaluate synaptic input in 72–96 hpf larvae. Burst duration was increased in tnt CaP neurons compared to that of sibling controls (for sibling control and tnt embryos, 8 and 6 cells were analyzed, respectively; p = 0.0001, two-tailed t-test). (C,D) To further assess the effects of decreased Eaat2 function, stimulated bursts were recorded before and after addition of the selective blocker DHKA. Addition of DHKA (160 µM) significantly increased burst duration (p = 0.004, two-tailed pair t-test), similar to the effect of the tnt mutation.

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Acknowledgments

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Reprinted from Developmental Biology, 362(2), McKeown, K.A., Moreno, R., Hall, V.L., Ribera, A.B., and Downes, G.B., Disruption of Eaat2b, a glutamate transporter, results in abnormal motor behaviors in developing zebrafish, 162-171, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.