ZFIN Image: Casano et al., 2016, Fig. S4

Image

Figure Caption

Fig. S4

Inhibition of nucleotide signaling impairs microglial precursors invasion in response to cell death. Related to Figure 4.

(A-D) Distribution (A-C) and quantification (D) of microglia in pU.1::GAL4-UAS::TagRFP embryos at 5.5dpf, 24h after DMSO, suramin and CBX treatment. Data from 2 independent experiments.

(E-H) Microglial distribution (E-G) and quantification (H) in the optic tectum of pU.1::GAL4- UAS::TagRFP embryos at 4.5dpf, 24 hours after drug washout. Data from 3 independent experiments.

(I-K) Dorsal view of mpeg1::GAL4-UAS::Kaede embryo showing invasion of macrophages after restoring nucleotide signaling. 24-hours after photoconversion and drug washout, macrophages that were present anteriorly are found inside the brain (red/yellow cells, white arrowheads).

(L) Microglial distribution in the optic tectum of pU.1::GAL4-UAS::TagRFP embryos at 2.5dpf, 24h after DMSO, suramin and CBX treatment. Data from 2 independent experiments.

(M-R) Distribution of microglial precursors in the anterior head region (upper panels) and optic tectum (lower panels) of the same slc7a7::Kaede embryos, 24h after drug treatment (M-O) or drug washout

(P-R). White arrowheads indicate slc7a7⁺ cells. Dotted lines delimit the anterior region of the head. Scale bar, 30µm. n, number of analyzed embryos. NS, non significant. *** p < 0.001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.