ZFIN Image: Just et al., 2016, Fig. S1

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Figure Caption

Fig. S1

Figure S1: Early cardiac development proceeds normally in png mutant embryos. Similar to wild-types (A, C), png mutant embryos (B, D) show a normal distribution of cmlc2 RNA at 24 and 48 hpf as revealed by whole-mount antisense RNA in situ hybridization. The heart regularly jogs to the left and loops to the right in wild-type (A, C) and png mutant (B, D) zebrafish embryos. (E-H) Hematoxylin/Eosin staining of sagittal histological sections of wt (E, G) and png mutant (F, H) hearts at 36 and 48 hpf, respectively. Similar to the wild-type situation (E, G), png mutant hearts (F, H) consist of normally developed endo- and myocardial cell layers. (I, J) Confocal images of Tg(fli1a:EGFP) transgenic wt (I) and png mutant (J) hearts at 72 hpf, showing that endocardial cushion cells fail to cluster at the AVC in png mutant hearts (marked with *). A, atrium; V, ventricle; my, myocardium; en, endocardium; ec, endocardial cushions.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.