ZFIN Image: Just et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Defective Tbx2b and not Wnt or Nodal signaling accounts for defective AVC development in png. (A, A′, B, B′) Heat-shock induced overexpression of Wnt8 to activate canonical Wnt signaling using the transgenic line Tg(hsp70l:wnt8a-GFP). Overexpression of wnt8 in wt does not phenocopy the png heart phenotype (n = 35, 3 independent experiments). (C, D) Ectopic expression of ndr2 in png mutants cannot rescue the heart phenotype (n = 29, 3 independent experiments). (E) Efficient expression of myc-tagged ndr2 in png mutants was demonstrated by Western-Blot. (F-I) In contrast to wt (F, H), tbx2b staining is absent in AVC of png mutants at 72 hpf (G, I). (J, L) tbx2b expression is reconstituted in med10 RNA-injected png mutants (L), but not in controls (J). (K, M) Accordingly, anf is misexpressed in AV myocardial cells of KCl-injected png mutants (K), whereas anf is absent from rescued AV myocardium of med10 RNA-injected png mutants (M) (white arrow, AVC).

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Acknowledgments

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